The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution.
Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.
The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.
The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution.
Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.
The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.
The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.
The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution.
Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.
The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.
The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution.
Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.
The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.
The µEncapsulator 2 Reagent Droplet Chip (hydrophilic) enables in vitro evolution, by forming millions of picolitre droplets that can be FACS–sorted. Applications include in vitro evolution of cells or viruses, or protein engineering by directed evolution.
Typically, clones from a sample such as an expression library, are singly encapsulated, together with a fluorogenic reaction mix, using the µEncapsulator 2 Reagent fluorophilic chip, to form water-in-oil droplets. These water-in-oil droplets are then re–encapsulated in an aqueous outer phase, using this µEncapsulator hydrophilic chip. The droplets would now have an aqueous core surrounded by an oil shell, in an aqueous continuous phase, and can be FACS–sorted.
The fluorophilic 2 Reagent Droplet Chip for the µEncapsulator module enables 2–reagent droplets containing biological material to be generated with ease. An output droplet size in the range Ø30µm –Ø70µm can typically be selected with high monodispersity. This chip interfaces directly with the Sample Reservoir Chip via the supplied FKM gasket. It can also be addressed independently for basic tests using the Multiflux® Linear Connector 4-way and Top Interface 4–way 2.15 mm. The 2 reagent streams meet immediately before the junction, minimising mixing prior to the formation of droplets. The Dolomite’s flow focussing junction design enables thousands of reactions per second to be carried out in volume-controlled compartments, isolated by a flowing carrier stream.
Part number 3200528Part number 3200527Part number 3200530Part number 3200529Part number 3200445Part number 3200564Part number 3200563Part number 3200566Part number 3200565Part number 3200568Part number 3200567
